Causes of mushroom spawn contamination

1.Mushroom sterilization is not complete, the cold air is not clear, the fire is not flourishing, the steam pressure is insufficient, the sterilization time is not enough.
2.The inoculation room or the vaccination box disinfection is not strict, the inoculation tool and the hand disinfection is not thorough。
3.Spawn bag is with micro holes,litter while bagging,not paying attention when handling.
4.The training room is not clean and sanitary, the humidity is too big, the spawn bottles’ and bags’ sealing is not strict, the temperature is too high, the ventilation is bad,and oxygen deficit.
5.Spawn culture material is not uniformly mixing, too much water, packed tightly, without good ventilation.
6.The spawn for inoculation switching is with infectious microbe.
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Method for producing shiitake mushroom stock culture and culture spawn

Method for producing shiitake mushroom stock culture and culture spawn
1.Sawdust culture medium:Broadleaf sawdust 78%, wheat bran 20%, sugar 1%, gypsum powder 1%, water content 55%-58%;
2.Cottonseed hull culture medium:cottonseed hull 78%,wheat bran 20%, sugar 1%, gypsum powder 1%, water content 55%-58%;
3.Corncob culture medium:Corncob coarse flour 80%, wheat bran 18%, sugar 1%, gypsum powder 1%, water content 55%-58%.Soak the crushed corncob corn grain size for 24 hours,remove them from water and dry them,mixing with wheat bran,sugar and gypsum powder,and then you can fill them in bags or bottles to sterilizing.
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Method for producing mushroom mother spawn

Common formula of edible fungus mother spawn culture medium：
1.edible fungus PDA culture medium:Potato 200 grams, glucose 20 grams, agar 20-23 grams, water 1000ml.

fermentation tank

2.Mushroom Integrated PDA culture medium:Potato 200 grams, glucose 20 grams , potassium dihydrogen phosphate 2 grams, magnesium sulfate 1.5 grams,vitamin B110mg, agar 20 grams and water 1000ml.
3.Shiitake mushroom Peptone - integrated PDA culture medium:add 2-3 grams peptone in the integrated PDA culture medium.

shiitake mushroom mother spawn making

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Raw and auxiliary materials

(1).Hard miscellaneous wood
Broad-leaved forest logs, branches, firewood is the main raw material of mushroom production.
(2).Processing wood sawdust
Wood sawdust is the basic raw material of mushroom production.
(3).Crop straw etc.
Grass stalks,corn cobs,cotton seed shell is the alternative materials for mushroom production.
(4).Fresh whole wheat bran
The main auxiliary materials for mushroom production
(5).Edible gypsum
Auxiliary materials for mushroom production
(6).Hydrated lime
It can adjust the substrate PH degree or used for field disinfection.
(7).Sucrose, potassium dihydrogen phosphate, magnesium sulfate
Auxiliary nutrition
(8).Polyethylene spawn bag
Close the culture materials,get into spawn column shape
(9).Non cotton cover body
Spawn bag sealing and deoxidization
(10).Spawn bottle
Spawn vessels
(11).Precision test paper
Test the substrates PH
(12).Alcohol, disinfectant, etc
Disinfect inoculation environment tools , sterilizing substrates .

Common facilities of mushroom

(1).Vaccination room for mushroom cultivating

Purpose:used for mother culture( first-generation culture medium),stock culture(second-generation culture medium) and cultivated culture(third-generation culture medium) inoculation.The requirement for the inoculation room is cleaning, ventilate, moisture-proof, regularly disinfect and sterilize.

(2).Inoculation box

Purpose:used for inoculation of mother culture( first-generation culture medium),stock culture(second-generation culture medium) and cultivated culture(third-generation culture medium) or cultivating bags.

(3).Temporary vaccination tent

Purpose:Mass production of cultivation bag inoculation .The requirement for the temporary inoculation tent is cleaning, ventilate, moisture-proof, regularly disinfect and sterilize. 

thermostat machine

(4).Strain cultivating room

Purpose:Cultivating mother culture( first-generation culture medium),stock culture(second-generation culture medium) and cultivated culture(third-generation culture medium) under constant temperature and away from light.Its requirement is cleaning, ventilate, moisture-proof, regularly disinfect and sterilize.

(5).Culture preservation room

Purpose:Mother culture preservation or stock culture and cultivated culture short-term storage.

(6). Plastic solar greenhouses

Purpose:Mass production and cultivation of cultivated culture and cultivated bags.

(7).The simple plastic greenhouses

Purpose:Cultivated bags fruiting production place

(8).Simple conjoined greenhouse

Purpose:Cultivated bags fruiting production place

(9).The fungus bag production greenhouses

Purpose:Materials mixing,bagging and sterilizing place for cultivated culture and cultivated bags

mushroom inoculation

(10).Mushroom Refrigerator

Short term storage and preservation of fresh mushroom

(11).Drying stove for mushroom

Purpose:Dried mushroom processing

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Main equipment for mushroom cultivating

(1).Horizontal high pressure sterilization machine    

    Its purpose is to sterilize stock culture( second-generation culture medium),culture spawn(third-generation culture medium),cultivating bags and so on culture medium filled in bags or bottles.

(2).Vertical high-pressure mushroom sterilizing machine       

    Its purpose is to sterilize mother species (first-generation) PDA test tube culture medium, inoculation tools etc.

(3).Large type high pressure steam boiler   

It is used for steam sterilizing for large and medium sized high pressure sterilizing machine.

mushroom sterilizing machine

(4).Small atmospheric pressure steam boiler

It is used for steam sterilizing for small atmospheric pressure steam boiler.

(5).Atmospheric pressure sterilization machine

It is used for normal pressure sterilization for culture medium and culture medium of culture bag

(6).Atmospheric opening type steaming pot

It is used for normally steaming semi - clinker cultivation 

(7).Atmospheric pressure steam sterilizer

    It is used for sterilizing culture medium of spawn bags and cultivating bags in normal pressure

steam sterilizer

(8).sawdust crusher

Its purpose is to crush log and then processed into wood raw material

(9).mixing machine

Its purpose is to mixing the raw materials

(10).Bag filling machine

Its purpose is to filling bags

(11).Mixing and bagging production line

Its purpose is to continuously complete the mixing and the bagging of the raw materials.

mushroom bagging production line

(12).Bag sealing machine

Its purpose is to seal bag

(13).Freezers

Its purpose is to preserve the mother culture and stock culture.

(14).Clean bench

Its purpose is to inoculate in small scale

mushroom clean bench

(15).Air conditioner and electric radiator

Its purpose is to help control the temperature of cultivating room

(16).The electric carrier vehicle

Its purpose is to carrying the spawns,cultivated bags and products.

(17).Plastic tray for mushroom cultivation

    Its purpose is to store the spawns,cultivated bags and products that need to handling.

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The whole life of Lentinula edodes

Lentinula edodes life cycle consists of two parts, namely the mycelium and sporocarp two stages.Mycelium period is the vegetative stage, sporocarp period is the reproductive growth stage.The sporocarp is called the mushroom fruit body, and the mushroom fruit body is the reproductive organ of the mushroom,consist of pileus and stipe two parts,the pileus back have  radial gills in which breed a large number of spores,it will ejected from gills when the spores mature.When the spores encounter appropriate nutrition, temperature and humidity, can generate mycelium germination,the mycelium just created is single-core mycelium, When confronted with different polarities mononuclear mycelia, can produce sexual combination,this combination is called sexual reproduction,also called heterothallic.The heterothallic mycelium breed cells through the lock-like joint,Within each cell contains twenty thousand nuclei, so called dual-core mycelium.When dual-core mycelium encounters the suitable nutrient and environmental conditions,it begin to differentiate to form the mushroom primordium,Continued growing and developing to grow into fruiting bodies.After fruiting bodies grow and mature, and breed a large amount of spores,that is the whole life of the shiitake mushroom.

delicious shiitake mushroom food

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Health care value of shiitake mushroom

The mushroom lentinan can enhance the immune function of body fluid.A large number of medical experiments proved that the lentinan the prevention and treatment of cancer has wide range , and has been used in clinical treatment.Mushrooms also contain a variety of vitamins and minerals,can promote human body, enhance the body's adaptability.Mushrooms also plays a role in treating diabetes, tuberculosis, infectious hepatitis, neuritis,and also can be used for indigestion, constipation, weight loss, etc.It is used to induce smallpox, measles and cure headache and giddiness in the folks.Mushrooms also contain double stranded RNA, can induce the production of interferon,with antiviral ability. Containing an element of reducing blood fat -,derivatives of adenine and adenine,is one of the rare health food.

mushroom food

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Nutritional value of shiitake mushroom

Dried mushrooms edible part accounted for 72%，Water content in the edible part is 13g per 100g,fat 1.8g,carbohydrate 54g, crude fibre 7.8g,ash content4.9g,calcium 124mg,Phosphorus 415mg, iron 25.3mg, vitamin B1 0.07mg, vitamin B2 1.13mg, nicotinic acid 18.9mg.

shiitake mushroom

Fresh shiitake mushroom except water content is 85%-90%,19% solids contains 19% crude protein,crude fat 4%,67% soluble nitrogen free substance,crude fibre 7%,ash content 3%.Shiitake mushroom are rich in vitamin D.The main aroma components of mushrooms are the mushroom essence,so the shiitake mushroom is also the important seasoning.

shiitake mushroom food

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Mushroom raw materials straw materials to cultivate mushroom

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Mushroom cultivation production line for view

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Sterilization machine for mushroom cultivation production line

 Sterilizer machine is classified into electric steam sterilizer ,electric oil sterilizer ,microwave or electric sterilizer according to its heating source.Both the two types are autoclave sterilizer.

sterilization machine

     Like this electric autoclave sterilizer is widely used for food industry, mushroom industry,medical industry etc.Most of all, it usually sterilize mushroom bag or mushroom bottle in the process of mushroom cultivation.We can also called it industrial sterilization machine.

sterilization machine

    Mushroom will become the food on the table as the improvement of people's living standard.Meanwhile, it become more popular in food industry.A complete mushroom sterilizer cover main body,recycle part,control part,safety part and record part.Each part play its own role in the whole sterilizing working process.

mushroom sterilizer

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